Quantification of microglial contact and engulfment of oligodendrocyte progenitor cells in the rodent brain

Quantification of microglial contact and engulfment of oligodendrocyte progenitor cells in the rodent brain

Blog Article

Summary: Microglia have emerged as essential regulators of neurodevelopment by phagocytosing synapses.Recently, we showed that microglia engulf viable oligodendrocyte progenitor cells (OPCs) during development to facilitate myelination.Here, we describe a protocol Seam Rippers to quantify microglial engulfment of whole cells using 3D confocal microscopy to differentiate microglial contact.This protocol can be applied to assess whole-cell engulfment in a variety of contexts and cell types.For complete details on the use and execution of this protocol, please refer Multi Cooker to Nemes-Baran et al.

(2020).